{"id":34490,"date":"2021-12-15T13:30:36","date_gmt":"2021-12-15T20:30:36","guid":{"rendered":"https:\/\/airsniper.ca\/?page_id=34490"},"modified":"2022-05-31T09:50:54","modified_gmt":"2022-05-31T15:50:54","slug":"airmid-test-results-for-in-line-unit-escherichia-virus-ms2","status":"publish","type":"page","link":"https:\/\/airsniper.ca\/test-results\/airmid-test-results-for-in-line-unit-escherichia-virus-ms2\/","title":{"rendered":"airmid Test Results for In-Line Unit: Escherichia virus MS2"},"content":{"rendered":"\t\t
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Airmid Final Laboratory Report: Escherichia virus MS2
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\n\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t<\/i>\t\t\t<\/span>\n\t\t\t\t\t\tDownload a PDF of This Report<\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Sample Description<\/td>Air Sniper Inflow Air Purifier<\/td><\/tr>
Number of Samples<\/td>1<\/td><\/tr>
Date of Receipt<\/td>05 October 2020<\/td><\/tr>
ASC Code<\/td>ASC004032<\/td><\/tr>
Report Number<\/td>ASCR092512<\/td><\/tr>
Report Date<\/td>01 October 2021<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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1. Purpose<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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To test the Air Sniper Inflow unit against aerosolised Escherichia virus MS2 in a multi-chamber setup consisting of two 28.5 m3<\/sup> environmental test chambers linked via ducting to allow for air to recirculate between the chambers.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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2. Test Item Description<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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The Air Sniper Inflow unit received by airmid healthgroup on 5th October 2020 was installed in an Environmental Test Chamber (Figure 2.1).<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Figure 2.1. Air Sniper Inflow unit installed inside the chamber at airmid healthgroup<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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3. Materials<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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3.1. Bacteriophage MS2 (MS2)<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Bacteriophage MS2 (MS2) is a non-enveloped virus that infects Escherichia coli <\/i>and some other closely related bacteria but has not been shown to infect eukaryotes. Like SARS-CoV- 2, MS2 is a single-stranded RNA virus. However, at approximately 27 nm in diameter, MS2 is much smaller than the 120 nm diameter SARS-CoV-2 virus. Because MS2 has similar aerosol characteristics to human viruses, it is often used in air purifiers and air filtration tests as a surrogate for viruses of similar or larger dimensions [1]. For example, MS2 has been used as a surrogate for Norovirus, including studies where MS2 has been aerosolised [2] and where viral inactivation by ultraviolet light has been assessed [3, 4]. MS2 is one of the bioaerosols recommended for air filtration tests by the EPA [5]. A study of the effect of UV exposure on virus aerosols found that MS2 was more resistant than the murine hepatitis virus (MHV) coronavirus to UV air disinfection [6]. Aerosols of the MHV coronavirus were found to be 7 \u2013 10 times more susceptible than MS2 [6]. Therefore, MS2 is a conservative surrogate for coronaviruses in this type of testing. However, as stated by the FDA: \u201c\u2026currently there is limited published data about the wavelength, dose, and duration of UVC radiation required to inactivate the SARS-CoV-2 virus<\/i>\u201d [7]. Based on the requirements for aerosolisation, the use of ultraviolet light as the antiviral technology and its suitability as a surrogate for some human viral pathogens, MS2 was used as the challenge microorganism in this study.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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3.2. 28.5 m3 Environmental Test Chambers<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Testing was conducted using two 28.5 m3<\/sup> test chambers purpose-built to comply with the American Society for Testing and Materials (ASTM) standard. The test chambers are connected via modular ducting (supply and return) and allow for recirculation of air between the two chambers. Both chambers have HEPA filtered supply air and can maintain selected temperature and humidity levels at a wide range of air change rates. The air change rate in the chambers can be controlled within a range of 0.5 to 20 air changes per hour. The chambers are constructed using powder-coated stainless steel with all materials complying with low volatile organic compound (VOC) emission requirements. Both chambers comply with cleanroom standards, are sealable from the exterior environment and are accessed via an anteroom with interlocking doors.<\/p>

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Chamber A \u2013 upstream, contained the Air Sniper inline unit and supply fan.<\/p>

Chamber B \u2013 downstream, contained a post-exposure duct section (Figure 3.1).<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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4. Procedure<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Six decay tests were performed as outlined below using a bespoke duct supplied by Air Sniper:<\/p>

Active Test:\u00a0<\/strong>conducted in triplicate\u00a0with<\/strong> the Air Sniper Inflow unit operating<\/p>

Inactive Control:\u00a0<\/strong>conducted in triplicate without the Air Sniper Inflow unit operating<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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